The Effect of Ethanol Extract Centella asiatica Leaves on Tumor NecrosisFactor Alpha (TNF-a) Levels in Rats Model of Pulpitis

Sari, Iin Indah The Effect of Ethanol Extract Centella asiatica Leaves on Tumor NecrosisFactor Alpha (TNF-a) Levels in Rats Model of Pulpitis. -. (Unpublished)

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Abstract

Pulpitis is an inflammatory condition of the dental pulp often triggered by bacterial infection. This inflammatory process involves various inflammatory mediators, including Tumor Necrosis Factor- Alpha (TNF-α), a key cytokine in the pathogenesis and progression of the disease. This study aimed to investigate the effect of administering ethanol extract of Centella asiatica leaves on TNF-α levels in the dental pulp of rats with induced pulpitis. Twenty-four male Sprague-Dawley rats, aged 8-10 weeks and weighing 150-200 grams, were randomly divided into four treatment groups: a negative control group (without pulpitis induction and treatment), a positive control group (with pulpitis induction and no treatment), and two treatment groups receiving different doses of ethanol extract of Centella asiatica leaves (e.g., 100 mg/kg body weight and 200 mg/kg body weight). Pulpitis was induced in the left mandibular first molar teeth of the rats by pulp exposure using a dental bur followed by Streptococcus mutans infection. The treatment groups received ethanol extract of Centella asiatica orally via gavage for 7 days. After 7 days of treatment, the dental pulp was extracted, and TNF-α levels were measured using the Enzyme-Linked Immunosorbent Assay (ELISA) method. Data were analyzed using One-Way ANOVA and Tukey's post hoc test. The results showed that the treatment group receiving the ethanol extract of Centella asiatica at a dose of 200 mg/kg body weight exhibited a significant reduction (p <0.05) in TNF-α levels compared to the positive control group. In conclusion, the ethanol extract of Centella asiatica at a dose of 200 mg/kg body weight has the potential to reduce TNF-α levels in the dental pulp of rats with pulpitis, suggesting its potential as an adjunctive therapeutic agent for pulpitis. Keywords: Centella asiatica, Ethanol Extract, TNF-α, Pulpitis, Dental Pulp, Rat, Conservative Dentistry

Item Type: Article
Subjects: R Medicine > RK Dentistry
Depositing User: Unnamed user with email rio.mahardiko@gmail.com
Date Deposited: 28 Mar 2025 03:09
Last Modified: 28 Mar 2025 03:09
URI: http://repository.unik-kediri.ac.id/id/eprint/729

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